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FluoroClean

Grid and chatter artifact correction for fluorescence microscopy

Images stay in this browser

Correction mode

Images

1 image

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Drop images or click to choose PNG · JPEG · WebP · TIFF · ND2

Live preview size

Auto preview

The preview uses the same FFT masks on a downscaled copy. Full output is processed separately and can retain the original pixel dimensions.

Output scale

Native XY preserves the original pixel dimensions. Large images automatically use memory-optimized FFT buffers; lower XY scales remain available for faster exports.

Parameters

Standard settings are ready to use. Open the technical controls only when an image needs manual tuning.

Correction limits100% = unrestricted

Maximum brightening100%

Maximum dimming100%

Each cap is measured against that channel's robust fluorescence range and is applied after the original FFT filter. Lower values constrain ringing or contrast shifts without changing the frequency mask.

Technical correction controls

Axis half-width2 px

Width of the horizontal and vertical frequency bands removed.

Mask feather1.5

Low-frequency protection8 px

Restores the FFT center to protect broad tissue intensity and contrast.

Auto-detect FFT angle

Manual FFT notch angle0°

This is the spectral line angle; visible stripes are approximately 90° rotated.

Notch half-width12 px

Mask feather12.0

Low-frequency protection8 px

Protect dark background

Mask threshold percentile2%

Export format

ND2 exports include every cleaned single channel, dual merge, and triple merge in the selected format.

Ready0%

Method notes

Grid mode uses the original first-iteration cross-axis FFT mask. Chatter mode uses the original first-iteration angle and frequency detection, targeted FFT suppression, and optional tissue mask. Live previews, original-resolution exports, and batch processing call those same masks. Very large transforms use 32-bit working buffers to reduce browser memory, and the optional brightening/dimming limits are applied only after filtering. Validate corrected intensities before quantitative analysis.

✦

Load a fluorescence microscopy image

Processing happens locally. Use the original FFT modes to target orthogonal grids or angled vibratome chatter.

Original

Corrected

No image loaded.

Supports ND2, PNG/JPEG/WebP, and TIFF. Multi-channel ND2 keeps source channels separate; dual merges use fixed green/magenta false colors and triples use fixed red/blue/green. Cleaned outputs can be exported as PNG or TIFF.